foot ulcer infection is one of the major complications of the diabetes patient.
Diabetic patients associated with foot ulcer have 10 fold greater risks in
hospitalized compared to without having diabetes. Widespread use and misuse of
antibiotic agents have resulted in problems of drug resistance linked to
treatment of infectious diseases caused by worrisome pathogens such as Acinetobacter baumannii
determine the prevalence of Antimicrobial susceptibility, ESBL and MBL producing
with comparing and correlate the expression of biofilm formation by Acinetobacter baumannii among diabetic
and non-diabetic foot ulcer patients.
Materials and Methods:
of 70 bacterial strains were obtained non-duplicate 35 strains of Acinetobacter baumannii in each among diabetic
foot ulcer (DFU) and non-diabetic foot ulcer(NDFU) which recovered 400 patients
from foot ulcers attending Yenepoya medical college hospital, Mangalore during
September 2016 to June 2017. Antimicrobial susceptibility testing by Kirby Bauer
disk diffusion method, Biofilm formation were determined using the Tissue
culture microtitre plate method, Extended-Spectrum ?-Lactamase (ESBL) and
Metallo- ?-Lactamase Production (MBL) by combined disk diffusion test, double
disk synergy test, and Modified Hodge test.
antimicrobial susceptibility profiles of NDFU were amikacin, ceftriaxone,
gentamicin, piperacillin/tazobactam, imipenem, meropenem, polymixin B,
tigecycline, and colistin were sensitive in Acinetobacter
baumannii and DFU were sensitive polymixin B, tigecycline, and colistin. In
our, study DFU isolates 13/35(37.14%) were ESBL, MBL 10/35(28.57%) and strongly
positive biofilm formation 9/35(25.71%). NDFU Isolates were ESBL 8/35(22.85%),
MBL 5/35(14.28%) and strongly positive biofilm formation 3/35(8.57%)
study highlight needs to establish antimicrobial susceptibility surveillance
for Acinetobacter baumannii to determine
the appropriate empirical treatment regimen and biofilm formation in DFU was 3
folds more severe threat to non-healing ulcer compared to NDFU patients.
colistin, tigecycline, and polymyxin B were the effective treatment against
multidrug-resistant pathogens. Acinetobacter
baumannii resistant to most classes of antibiotics was continue to emerge
unless inappropriate uses of drugs are curtailed and continuous education of
infection control practices maintained with ulcer management in the hospital